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Online Course – 1st Edition

Introduction to DiceCT: Diffusible Iodine-based Contrast-enhanced Computed Tomography

August 23rd – September 15th, 2021




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This course will be delivered live online

This course will be taught with live (synchronous) sessions


Course Introduction to DiceCT - Diffusible Iodine-based Contrast-enhanced Computed TomographyCourse overview

This DiceCT course is about staining specimens for x-ray contrast enhancement for CT imaging, with iodine-based staining agents. We will focus on iodine contrast stains, their preparation in the lab, staining times and precautions for different specimen types and sizes, protocols for staining and de-staining, and alternative contrast agents like phosphotungstic acid. Specific sample types of interest to you will be discussed. In the beginning, we will also briefly touch on the basics of microCT imaging. In the interval between the two parts of the course, ideally, course participants will stain and CT scan their own material, and we will discuss results and protocols in the second part. If participants do not have access to it, then different examples will be discussed.

This interactive online course is primarily aimed at researchers planning or beginning to use contrast-enhanced (micro)CT imaging in their own work. Ideally, if you have access to a CT or microCT, the second part of the course is for discussing your own imaging results, successes, and problems with the group. We will look at examples of properly and improperly stained materials and talk about troubleshooting strategies.

Check here the full program


All participants must have a personal computer (Windows, Macintosh), with webcam if possible, and a good internet connection.




August 23rd – September 15th, 2021

Schedule and Course length

Online live sessions on 23rd, 24th, and 25th of August, and on 14th and 15th of September;  from 15:00 to 18:30 (Madrid time zone).

17.5 hours




Places are limited to 16 participants and will be occupied by strict registration order.

Participants who have completed the course will receive a certificate at the end of it.

Anna Nele Herdina instructor for Transmitting Science

Dr. Anna Nele Herdina
Medical University of Vienna

Paul Gignac instructor for Transmitting Science

Dr. Paul Gignac
Oklahoma State University Center for Health Sciences

Jordi Galbany Transmitting Science Coordinator

Dr. Jordy Galbany

Transmitting Science

Haris Saslis coordinator for Transmitting Science

Dr. Haris Saslis
Transmitting Science


23rd of August, 2021 – General overview microCT imaging (Dr. Anna Nele Herdina)

  • x-ray imaging basic principles
  • microCT imaging and settings
  • image analysis

24th of August, 2021 – Contrast enhancing for microCT (Dr. Anna Nele Herdina)

  • staining and de-staining agents
  • lab preparation and precautions
  • the risk to specimens
  • working with natural history museum collections

25th of August, 2021 – Contrast enhancing for microCT (Dr. Anna Nele Herdina and Dr. Paul Gignac)

  • protocols for specific sample types
  • planning for your specific sample types
  • how to set up your staining lab

(If you have access to a CT or microCT: staining and scanning first samples; with e-mail or slack-channel assistance, this is optional)

14th of September, 2021 – Discussion of imaging results (Dr. Anna Nele Herdina)

  • how to determine if the specimen was stained for the right time
  • specimen damage
  • de-staining
  • discussion of course participant results and other examples

15th of September, 2021 – Discussion of imaging results (Dr. Anna Nele Herdina)

  • discussion of course participant results and other examples
  • General discussion and further protocol planning


  • Course Fee
  • Early bird (until July 30th, 2021):
  • 410 €
    (328 € for Ambassador Institutions)
  • Regular (after July 30th, 2021):
  • 490 €
    (392 € for Ambassador Institutions)
  • The price is VAT included.
    After registration you will receive confirmation of your acceptance in the course. Payment is not required during registration.

You can check the list of Ambassador Institutions. If you want your institution to become a Transmitting Science Ambassador please contact us at communication@transmittingscience.com


Discounts are not cumulative and apply only on the Course Fee. We offer the possibility of paying in two instalments (contact courses.crete@transmittingscience.org).

Former participants will have a 5 % discount on the Course Fee.

20 % discount on the Course Fee is offered for members of some organizations (Ambassador Institutions). If you want to apply to this discount please indicate it in the Registration form (proof will be asked later).

Unemployed scientists living in Spain, as well as PhD students based in Greece without any grant or scholarship to develop their PhD, could benefit from a 40 % discount on the Course Fee. If you want to ask for this discount, please contact the course coordinator. That would apply for a maximum of 2 places and they will be covered by strict inscription order.