This course will be taught with live (synchronous) sessions
This DiceCT course is about staining specimens for x-ray contrast enhancement for CT imaging, with iodine-based staining agents. We will focus on iodine contrast stains, their preparation in the lab, staining times and precautions for different specimen types and sizes, protocols for staining and de-staining, and alternative contrast agents like phosphotungstic acid. Specific sample types of interest to you will be discussed. In the beginning, we will also briefly touch on the basics of microCT imaging. In the interval between the two parts of the course, ideally, course participants will stain and CT scan their own material, and we will discuss results and protocols in the second part. If participants do not have access to it, then different examples will be discussed.
This interactive online course is primarily aimed at researchers planning or beginning to use contrast-enhanced (micro)CT imaging in their own work. Ideally, if you have access to a CT or microCT, the second part of the course is for discussing your own imaging results, successes, and problems with the group. We will look at examples of properly and improperly stained materials and talk about troubleshooting strategies.
All participants must have a personal computer (Windows, Macintosh), with webcam if possible, and a good internet connection.
Click here to see the full Program
23rd of August, 2021 – General overview microCT imaging (Dr. Anna Nele Herdina)
24th of August, 2021 – Contrast enhancing for microCT (Dr. Anna Nele Herdina)
25th of August, 2021 – Contrast enhancing for microCT (Dr. Anna Nele Herdina and Dr. Paul Gignac)
(If you have access to a CT or microCT: staining and scanning first samples; with e-mail or slack-channel assistance, this is optional)
14th of September, 2021 – Discussion of imaging results (Dr. Anna Nele Herdina)
15th of September, 2021 – Discussion of imaging results (Dr. Anna Nele Herdina)