Course – 2nd Edition
Introduction to Electron Microscopy for Life Sciences
July 4th-7th, 2017, Barcelona (Spain)
This course gives an introduction to both Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM). It covers electron microscopy principles and theory, as well as different fields of application, operation modes and image interpretation.
It is aimed at beginners and intermediate users of electron microscopes in life sciences. The course has a limited number of participants to allow small groups during practical sessions.
After this course, the participants should be able to align the microscopes, identify the most common aberrations and understand the principles of image generation in electron microscopy. Participants can also bring some samples already prepared on TEM grids or SEM stubs.
September 9th-13th, 2019
26 hours on-site.
This course is equivalent to 1 ECTS (European Credit Transfer System) at the Life Science Zurich Graduate School and ForBio.
The recognition of ECTS by other institutions depends on each university or school.
Places are limited to 12 participants and will be occupied by strict registration order.
Participants who have completed the course will receive a certificate at the end of it.
Tuesday, July 4th, 2017.
Theory. Introduction to optics. Fundamentals of electron microscopy. Electromagnetic lenses. Vacuum systems. Electron guns. Scanning Electron Microscope (SEM). Columns and lenses. Image detectors. SEM principles.
Wednesday, July 5th, 2017.
Practical session. SEM and TEM basics I.
Theory. SEM. Energy Dispersive X-Ray Spectroscopy (EDS), Catodoluminiscence, Environmental SEM. Low voltage SEM, High Resolution SEM, non-conductive samples. Examples and applications. Sample preparation for SEM. Conventional method and immunolabeling. Critical point drying and surface coatings. Correlative microscopy.
Thursday, July 6th, 2017.
Practical session. SEM and TEM basics II.
Theory. Transmission Electron Microscope (TEM). Columns and lenses. Detectors. Electron Energy Loss Spectroscopy (EELS), Energy Filtered TEM (EFTEM) and High Angle Anular Dark Field (HAADF). Cryo TEM and Tomography. Sample preparation for TEM (conventional methods, cytochemistry, and cryomethods). Embedding and sectioning. Immunolabelling. Vitrification for cryo EM.
Friday, July 7th, 2017.
Practical session. SEM and TEM advanced I.
Practical session. SEM and TEM advanced II.
You can check the list of Ambassador Institutions. If you want your institution to become a Transmitting Science Ambassador please contact us at firstname.lastname@example.org
The class schedule is approximate; it is possible that the content of one day may run into the next and a working day may be longer than advertised.
The course will take place in the city of Sabadell, Barcelona (Spain). Take a look at the venue.
You may stay in Barcelona city or Sabadell. You will find information about Hotels and Hostel in Sabadell here. It takes about 45 minutes by public transport to arrive to Sabadell from the centre of Barcelona city. The place of the course is about 15 minutes walking from the train stop.
Discounts are not cumulative and apply only on the Course Fee. We offer the possibility of paying in two instalments (contact email@example.com).
5 % Discount
Former participants will have a 5 % discount on the Course Fee.
20 % Discount
20 % discount on the Course Fee is offered for members of some organizations (Ambassador Institutions). If you want to apply to this discount please indicate it in the Registration form (proof will be asked later).
40 % Discount
Unemployed scientists living in Spain, as well as PhD students based in Spain without any grant or scholarship to develop their PhD, could benefit from a 40 % discount on the Course Fee. If you want to ask for this discount, please contact the course coordinator. That would apply for a maximum of 2 places and they will be covered by strict inscription order.