This course gives an introduction to both Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM). It covers electron microscopy principles and theory, as well as different fields of application, operation modes and image interpretation.
It is aimed at beginners and intermediate users of electron microscopes in life sciences. The course has a limited number of participants to allow small groups during practical sessions.
After this course, the participants should be able to align the microscopes, identify the most common aberrations and understand the principles of image generation in electron microscopy. Participants can also bring some samples already prepared on TEM grids or SEM stubs.
Tuesday, July 4th, 2017.
Theory. Introduction to optics. Fundamentals of electron microscopy. Electromagnetic lenses. Vacuum systems. Electron guns. Scanning Electron Microscope (SEM). Columns and lenses. Image detectors. SEM principles.
Wednesday, July 5th, 2017.
Practical session. SEM and TEM basics I.
Theory. SEM. Energy Dispersive X-Ray Spectroscopy (EDS), Catodoluminiscence, Environmental SEM. Low voltage SEM, High Resolution SEM, non-conductive samples. Examples and applications. Sample preparation for SEM. Conventional method and immunolabeling. Critical point drying and surface coatings. Correlative microscopy.
Thursday, July 6th, 2017.
Practical session. SEM and TEM basics II.
Theory. Transmission Electron Microscope (TEM). Columns and lenses. Detectors. Electron Energy Loss Spectroscopy (EELS), Energy Filtered TEM (EFTEM) and High Angle Anular Dark Field (HAADF). Cryo TEM and Tomography. Sample preparation for TEM (conventional methods, cytochemistry, and cryomethods). Embedding and sectioning. Immunolabelling. Vitrification for cryo EM.
Friday, July 7th, 2017.
Practical session. SEM and TEM advanced I.
Practical session. SEM and TEM advanced II.
- Course Fee
- Early bird (until July 31st, 2019):
- 540 € *
(432 € for Ambassador Institutions)
- Regular (after July 31st, 2019):
- 675 € *
(540 € for Ambassador Institutions)
- This includes course material, coffee breaks and lunches (VAT included).
* Participants from companies/industry will have an extra charge of 100 €.